cd68 ed1  (Bio-Rad)

Journal: Diseases

Article Title: Hypovitaminosis D Does Not Aggravate the Progression of Gentamicin-Induced Kidney Injury in Rats

doi: 10.3390/diseases13070200

Figure Lengend Snippet: ( A ) Immunolocalization of CD68+ cells in renal cortex of rats from protocols 1 (5 days) and 2 (30 days) fed with standard diet (VitD) or VitD-free diet (VitD — ) treated with saline (Ctrl) or gentamicin (Genta). Magnification 400×. Quantification of the number of CD68+ cells obtained from protocol 1 ( B ) and protocol 2 ( C ). Data are expressed as mean ± SEM. d p < 0.05 vs. Ctrl VitD; g p < 0.01, h p < 0.05 vs. Ctrl VitD — .

Article Snippet: Then, we incubated the renal tissues with the following primary antibodies: anti-vimentin (1/500, MO 725, Dako Corporation, Glostrup, Denmark) for 60 min at room temperature; anti-ED1 (CD68) (1/1000, MCA341R, Serotec, Kidlington, UK); and anti-fibronectin (1/500, Chemicon International Inc., Temecula, CA, USA) incubated overnight at 4 °C.

Techniques: Saline